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Image Search Results
Journal: Translational Stroke Research
Article Title: Remote Ischemic Preconditioning Exerts Neuroprotective Effects Via the PGC-1α/FNDC5/BDNF Pathway in Focal Brain Ischemia of Rats
doi: 10.1007/s12975-026-01422-z
Figure Lengend Snippet: Effects of RIpreC on the expression of Bcl-2 ( A , B , F , G ), Bax ( A , C , F , H ), caspase-3 ( A , D , F , J ), and TUNEL-positive neurons ( A , E ) in the ischemic brain. Bcl-2 immunoreactivity in the perilesional cortex and its protein level were significantly increased in the RIpreC group compared the IR group. By contrast, Bax immunoreactivity and its protein level were significantly reduced in the RIpreC group compared with the IR group. The Bax/Bcl-2 ratios were also significantly reduced in the RIpreC group compared with the IR group ( I ). Subsequently, the number of TUNEL-positive neurons (arrow) and the ratios of caspase-3 positive to NeuN-positive neuron in the perilesional cortex were significantly decreased in the RIpreC group compared with the IR group ( E , K , L ). Mean ± standard error. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Scale bar = 50 μm ( A ) and 25 μm ( K ). ( n = 4–11 in each group)
Article Snippet: The following primary antibodies were used: rabbit anti-PGC-1α (1:1000; ab191838; Abcam plc, Cambridge, UK), rabbit anti-FNDC5 (1:1000; 23995-1-AP; Proteintech), rabbit anti-BDNF (1:500; ab108319; Abcam plc, Cambridge, UK), anti-rabbit-phospho-Akt (1:1000; #9271; Cell Signaling), anti-rabbit-Akt (1:1000; #9272; Cell Signaling),
Techniques: Expressing, TUNEL Assay